RESTSITE v1.2                                         March, 1991

               RESTSITE  v1.2  is  a program  that  will  calculate genetic
          distance between OTUs  from THE ORIGINAL DATA, not  from a matrix
          of sites or fragments.  Programs included are:

          READTEXT - reads  ASCII text files where each  line contains data
                     from one  individual cut  with one  enzyme and  probed
                     with one  probe (one "lane").   A binary data  file is
                     produced.
          WRITETXT - reads  binary data  files and converts them  back into
                     ASCII.  This aids portability to other machines.
          RESTSITE - reads  binary  data files, groups  the data  into OTUs
                     specified by the user, extracts the sites/fragments in
                     common, and  calculates the distance between the OTUs.
                     Also produces distance matrices.
          REPORT   - generates a listing of the data found within each OTU,
                     broken down by enzyme and probe.
          CALCD    - a  stand-alone   program  that  computes   d-hat   via
                     equations  5.53-5.55  of Nei  (1987),  when given  the
                     fragments found in OTU X, Y, and in common.
          REMFILE  - temporarily removes an OTU from the study.
          COMBINE  - temporarily combines two OTUs into one.
          REMDATA  - temporarily  removes  the data found  in one OTU  from
                     another  OTU   (useful  in   finding  species-specific
                     sites/fragments).
          01_TO_RS - converts 0/1 data matrices to RESTSITE format.
          RS_TO_01 - converts RESTSITE data to 0/1 matrices.
          NJTREE   - constructs   Neighbor-Joining   trees   from  distance
                     matrices.
          UPGMA    - constructs UPGMA trees from distance matrices.

               Documentation  files,  source  code, and  header  files  are
          included.  The programs are written in Microsoft C v5.01, and are
          compiled for the MS-DOS operating system.

               I did everything I could  to extensively test these programs
          on a variety of data, but I  am not omniscient, and cannot forsee
          all types of data and all problems that people are going to have.
          If you do have  problems, please contact me.  A copy of your data
          will probably help.

               Copies  of this  package  can  be obtained  from  me at  the
          address below.   Please  send me one  of the  following types  of
          diskettes (formatted, please):
                    -- two  5-1/4" high-density diskettes (1.2Mb), 
                    -- four 5-1/4" double-sided, double-density diskettes,
                    -- one  3-1/2" diskette (1.44Mb).
          A  self-addressed, stamped  envelope (large  enough  to hold  the
          disks) is not necessary, but appreciated.

               If you have any suggestions  that would improve the  clarity
          and usefulness of these programs, please let me know.  Thank you.

                                Dr. Joyce C. Miller   
                                Whitehead Institute   
                                9 Cambridge Center    
                                Cambridge, MA 02142   
                                (617) 253-8582