Platforms

Proteomics Platform

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Scientists in the Proteomics platform seek to provide comprehensive tools for understanding the expression, activation, interaction and signaling of proteins and metabolites. The Proteomics platform has a wealth of expertise in protein chemistry, separations science, mass spectrometry and next-generation approaches for data analysis. These capabilities, together with the use of state-of-the-art instrumentation (such as a new hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometer), provide the essential ingredients for rapid and successful proteomic discovery.

Platform scientists collaborate with other Broad scientists and the biomedical research community to design and execute large-scale protein profiling projects. Scientists in the platform also develop new methods to improve detection and quantitation of proteins and their modifications. The platform is directed by Steve Carr, an expert in proteomics and mass spectrometry. Other platform leaders include Terri Addona, Karl Clauser and Michael Gillette.

The platform's major activities include:

Biological sample analysis

This work includes quantitative analysis of differentially expressed proteins and metabolites derived from complex biological specimens (such as cell lines, tumor tissues or patient blood) to identify new biomarkers of disease. In addition, platform scientists are undertaking phosphoproteomics studies to develop signatures of kinase activation, and to produce novel insights into pathogenesis, signaling pathways, and drug mechanism.

Small molecule targets

These efforts include metabolic profiling and identification of the protein targets of small molecules. Understanding the mechanisms of action of small molecules has significant implications for both basic research and drug discovery. In addition, platform scientists are initiating quantitative metabolic profiling studies of small molecules aimed at discovering non-protein biomarkers of heart disease and inflammation.

Header image courtesy of Molecular & Cellular Proteomics 3:478-489, 2004.

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Proteomics Platform

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