Sequenom MassArray
Specifications at a glance
| hME | iPLEX | |
|---|---|---|
| Fixed/Custom | Custom | Custom |
| SNPs/ng DNA | 1.0 | 2.4 |
| DNA input (ng) | 5 | 10 |
| Start-up time (wk) | 1 | 3 |
| Study duration (wk) | 2 | 4 |
Parameter estimates are based on a 10-plate (940 sample) study, and on current platform capacity, and assume immediate availability and usability of a customer’s SNP list and DNA samples. These parameters are for comparison and planning purposes, and are not necessarily reflective of actual results. Time measures reflect laboratory time, and not post-lab data analysis. The above examples assume a 5-plex hME or a 24-plex iPLEX SNP assay design.
Platform Description
Sequenom SNP genotyping uses a bead-less and label-free primer-extension chemistry to generate allele-specific products with distinct masses. The backbone of high-throughput detection is the speed and accuracy of Matrix-Assisted Laser Desorption / Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS). Allele discrimination is conferred by primer extension with a high-fidelity polymerase enzyme across the SNP site, leading to differences in mass of extended products. Differentiation of genotypes for each of the SNPs multiplexed in one assay is a result of unique mass ranges for the extension primers.
CGA currently offers Sequenom’s hME technology, allowing the rapid design and deployment of pooled assays with up to seven SNPs, and which optionally include validation of each assay on a well-characterized sample set. Soon to be available is Sequenom’s higher complexity iPLEX technology, wherein on the order of twenty SNPs are simultaneously assayed.
Services offered
hME: any number of custom SNPs in pooled assays of up to 7 SNPs
iPLEX: any number of custom SNPs in pooled assays of up to 24 SNPs
Cost
Please refer to this page for current prices.
Details...
SEQUENOM Technology
Sequenom technology combines a homogeneous reaction format with standardized assay conditions. Each assay has a single extension primer to interrogate both alleles, minimizing the total number of reactions required.
The MassEXTEND reaction has three levels of biological stringency:
- Polymerase chain reaction (PCR) target amplification
- Extension primer binding
- Primer extension across polymorphic base(s) with a high-fidelity DNA polymerase enzyme
- For further information, please visit Sequenom's website.
Contact
Contact information may be found here.