The RNAi Consortium shRNA Library

• The RNAi Consortium, or TRC, is a public-private effort based at the Broad whose mission is to create a shRNA library as well to validate tools and methods that will enable the scientific community to use RNAi to determine the function of human and mouse genes. The reagents are composed of short hairpin sequences carried in lentiviral vectors arrayed in 96-well plates.

Library Distribution

• The TRC shRNA library is distributed as bacterial glycerol stocks, plasmid DNA or lentiviral particles by Sigma-Aldrich and as bacterial glycerol stocks by Open Biosystems. The Broad RNAi platform does not directly distribute the TRC library.

Search for Available TRC shRNA Constructs

• Sigma-Aldrich
• Open Biosystems
• TRC library database

shRNA Target Selection Rules and Genes Targeted

• Description of the candidate-picking process and rules
• human genes targeted as of April 2007
• mouse genes targeted as of April 2007
• Contents of the TRC library as of Oct 2006 (4.2 Mb bzip2 compressed file)

Library Performance Data

Date and Figures	Date	Download
Interferon response genes are not induced following transduction with TRC Lentiviral shRNAs	14 Jul 2006	pdf

Protocols and Vectors

• Protocols

Select Publications From Labs Employing the TRC shRNA Library

• Publications