Genetic, Physical, and Optical Maps
The Sclerotinia Optical Map was constructed by OpGen using the restriction enzyme BsiWI. The map consists of 16 linkage maps, which appear to correspond to chromosome units. The map labeled ChrR is linked to rDNA repeats, which are found in scaffold 35 (unmapped). An in silico restriction digest of Sclerotinia assembly was generated to compare the assembly to the optical map. Correspondence of the restriction enzyme cutting sites and the predicted fragment lengths were used to order and orient the scaffolds of the Sclerotinia assembly to the optical map. This procedure anchored more than 99% of the assembly to the optical map; the 34 anchored scaffolds cover 99% of the optical map. Note that scaffold 4 aligns to 2 optical contigs; inspection of the assembly supports that there is a mis-join in scaffold 4 between contigs 132 and 133.
A poster-sized version of the physical-optical maps is available. Click on the image below to download it.
